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Journal of Lipid Research, Vol. 8, 515-518, September 1967
Copyright © 1967 by Lipid Research, Inc.

Internal standards in the estimation of acetyl-CoA in liver extracts

Emilio Herrera and Norbert Freinkel

Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611

The necessity for adding an internal standard to liver extracts during fluorimetric estimation of acetyl-CoA by the malic dehydrogenase-citrate synthase reaction is demonstrated. Addition of acetyl-CoA completely compensates for the inhibitory action of some tissue components. Values for hepatic acetyl-CoA in fed and fasted rats are given.

Supplementary key words acetyl-CoA • fluorimetric assay • rat • liver • fasting

Submitted on April 24, 1967
Accepted on June 14, 1967


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