J. Lipid Res.
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Journal of Lipid Research, Vol. 8, 565-568, November 1967
Copyright © 1967 by Lipid Research, Inc.

Quantitative gas-liquid chromatographic analysis of ethanolamine, monomethyl ethanolamine, and dimethyl ethanolamine from lipids

Robert L. Lester and David C. White

Department of Biochemistry, University of Kentucky Medical Center, Lexington, Kentucky 40506

A method for the separation and quantitative estimation of ethanolamine, dimethyl ethanolamine, and monomethyl ethanolamine derived from lipids is described. After acid hydrolysis of the lipids, the bases are extracted from the neutralized hydrolysate with t-butonol and separated by gas-liquid chromatography.

Supplementary key words dimethyl ethanolamine • ethanolamine • monomethyl ethanolamine • gas-liquid chromatography • phospholipids • quantitative assay

Submitted on May 12, 1967
Accepted on July 5, 1967


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