Journal of Lipid Research, Vol. 8, 667-675, November 1967
Copyright © 1967 by Lipid Research, Inc.

Composition of phospholipids and of phospholipid fatty acids and aldehydes in human red cells

James T. Dodge and Gerald B. Phillips

Department of Medicine, College of Physicians and Surgeons, Columbia University, New York 10032

Improved methods for lipid analysis that have been developed recently were employed to reevaluate the phospholipid composition, the fatty acid and fatty aldehyde composition of the total phospholipid, and the fatty acid composition of the individual phospholipids of normal human red cells.

Thirty-three fatty acids and five fatty aldehydes were estimated and tentatively identified in the total phospholipid of normal human red cells. Additional minor components were evident.

The major individual phospholipids were isolated by silicic acid thin-layer chromatography and quantified. The fatty acid compositions of phosphatidyl ethanolamine, phosphatidyl serine, lecithin, and sphingomyelin were determined. Each of these phospholipids showed a distinctive and characteristic fatty acid pattern.

Supplementary key words phospholipids • fatty acids • fatty aldehydes • human red cells • phosphatidyl ethanolamine • phosphatidyl serine • lecithin • sphingomyelin • autoxidation

Submitted on May 1, 1967
Accepted on August 14, 1967

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