Journal of Lipid Research, Vol. 9, 1-7, January 1968
Copyright © 1968 by Lipid Research, Inc.

Hepatic triglyceride secretion in relation to lipogenesis and free fatty acid mobilization in fasted and glucose-refed rats

Nome Baker , Arlene S. Garfinkel , and Michael C. Schotz

Radioisotope Research, Veterans Administration Center, Los Angeles, California 90073, and Department of Biological Chemistry, University of California at Los Angeles School of Medicine, Los Angeles, California 90024

Plasma triglyceride concentrations were significantly lowered by a single feeding of glucose to rats that had been fasted for 22 hr. Three feedings of glucose produced a similar effect. In the glucose-refed animals mobilization of free fatty acids from adipose tissue was impaired more rapidly than hepatic lipogenesis was restored from its low fasting level. These effects of glucose were shown by both a 50% fall in plasma free fatty acid concentration and an 84% decrease in free fatty acid release by isolated epididymal fat pads within 30 min after a single refeeding of glucose. Hepatic lipogenesis from either acetate-1-¹⁴C or glucose-U-¹⁴C was not restored even after glucose had been fed three times at hourly intervals. Triton-induced hypertriglyceridemia was used to measure the hepatic triglyceride secretory rate; it was found that glucose refeeding decreased this rate in all but one of several experiments. This decreased secretion rate was sufficient to account for the nearly complete disappearance of triglyceride in very low density lipoproteins (d < 1.019) that occurred within 1 hr after a single glucose intubation.

Supplementary key words rat • fasting • glucose refeeding • triglycerides • free fatty acids • mobilization • hepatic lipogenesis • Triton-induced hypertriglyceridemia • ether anesthesia • very low density lipoprotein

Submitted on May 10, 1967
Accepted on August 28, 1967

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