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Journal of Lipid Research, Vol. 9, 110-119, January 1968
Copyright © 1968 by Lipid Research, Inc.
The Rockefeller University, New York 10021
Four methods for the sizing of adipose cells in small samples of human or animal adipose tissue are compared. These methods depend on the preparation of cell suspensions by incubation of the tissue with collagenase or by prolonged fixation with osmium tetroxide and separation of the fixed cells. A Coulter electronic counter was used to count and size the suspended cells and a Zeiss particle size analyzer for the sizing of cells in photomicrographs. The use of the Coulter counter to count cells in a suspension derived from a known amount of tissue and subjected to osmium tetroxide fixation is recommended for accuracy and general applicability to adipose cells of all sizes in man and animals.
Supplementary key words adipose tissue • adipose cell • cell size • cell number • cell suspensions • electronic counting • Coulter counter • cell fixation • osmium tetroxide • collagenase
Submitted on August 4, 1967
Accepted on October 13, 1967
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