J. Lipid Res.
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Journal of Lipid Research, Vol. 9, 146-148, January 1968
Copyright © 1968 by Lipid Research, Inc.

Bis(trimethylsilyl)acetamide in the silylation of lipolysis products for gas-liquid chromatography

W. H. Tallent and R. Kleiman

Northern Regional Research Laboratory, Peoria, Illinois 61604

When bis(trimethylsilyl)acetamide is used to silylate lipolysates for gas-liquid chromatography, the pyridine solution containing the sample and silylating agent can be injected directly onto the column. Conversion of free fatty acids to methyl esters before silylation is unnecessary, since silyl esters elute as sharp gas-liquid chromatographic peaks.

Supplementary key words silylation • trimethylsilylation • silyl esters • bis(trimethylsilyl)acetamide • hexamethyldisilazane • trimethylchlorosilane • gas-liquid chromatography • pancreatic lipase • intraglyceride distribution patterns • vernolic acid • dimorphecolic acid • agr-eleostearic acid • Cephalaria joppica • Dimorphotheca sinuata • Centranthus macrosiphon

Submitted on August 14, 1967
Accepted on September 14, 1967


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