J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 9, 8-11, January 1968
Copyright © 1968 by Lipid Research, Inc.

Isolation from yeast of a metabolically active water-soluble form of ergosterol

B. G. Adams and L. W. Parks

Department of Microbiology, Oregon State University, Corvallis, Oregon 97331

A water-soluble complex containing ergosterol together with a component of yeast has been isolated. The complex can be isolated from commercial yeast extract to which ergosterol has been added or directly from whole yeast cells. The complexing component has the properties of a large polysaccharide and the binding between the sterol and the polysaccharide appears to be noncovalent. The complex is easily prepared and is stable in aqueous solution; ergosterol in this solution is metabolically available to yeast cells to which it is added.

Supplementary key words ergosterol • soluble form • complex • yeast extract • digitonide • dimethyl sulfoxide

Submitted on June 27, 1967
Accepted on September 11, 1967


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