Journal of Lipid Research, Vol. 9, 93-97, January 1968
Copyright © 1968 by Lipid Research, Inc.

Identification of the fecal metabolites of 17-methyltestosterone in the dog

Erwin H. Mosbach , Sarah Shefer , and Liese L. Abell

The Public Health Research Institute of the City of New York, Inc., and The Bureau of Laboratories, New York City Department of Health; and Columbia University Research Service, Goldwater Memorial Hospital, and the Departments of Biochemistry and Medicine, Columbia University College of Physicians and Surgeons, New York 10027

17-Methyltestosterone-4-¹⁴C was fed to two dogs in an experiment to determine tissue localization and metabolic disposition of this hypocholesterolemic steroid. No accumulation of the drug was found in any tissue, although a small amount of radioactivity was detected in the liver and the ileal mucosa of one animal. Most of the administered radio-activity was excreted in urine and feces.

The urinary metabolites consisted largely of highly polar compounds which appeared resistant to glucuronidase treatment or solvolysis procedures. Analysis of the fecal metabolites showed the presence of unchanged methyltestosterone, of four isomeric methylandrostanediols, and of labeled unidentified polar compounds. Of the four identified methylandrostane-diols, the predominating fecal diols were 17-methyl-5-androstane-3ßbeta;,17ßbeta;-diol (45-62%) and 17-methyl-5ßbeta;-androstane-3,17ßbeta;-diol (12-28%); 17-methyl-5-androstane-3,-17ßbeta;-diol and the 5ßbeta;:3ßbeta; isomer were found in very small amounts only.

Supplementary key words 17-methyltestosterone • metabolism • dog • excretion • 17-methylandrostanediols • gas-liquid • column • chromatography

Submitted on July 31, 1967
Accepted on October 6, 1967

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