J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 9, 176-179, March 1968
Copyright © 1968 by Lipid Research, Inc.

Characterization of vitamin K from bovine liver

John T. Matschiner and Josephine M. Amelotti

Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104

Concentrated fractions of vitamin K from bovine liver were purified by thin-layer chromatography and fractions were analyzed by UV spectroscopy and mass spectrometry. The chromatographic behavior of the purified vitamins was compared with that of known compounds on thin layers of silica gel, either untreated or impregnated with silver nitrate or paraffin.

The principal forms of vitamin K recovered from bovine liver were highly lipophilic. Two fractions were obtained which collectively gave identifiable mass spectra of menaquinone-10, menaquinone-11, and menaquinone-12.

Supplementary key words vitamin K • bovine liver • mass spectrometry • lipophilic naphthoquinones • thin-layer chromatography • menaquinones-10, 11, 12

Submitted on September 5, 1967
Accepted on October 24, 1967


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