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Journal of Lipid Research, Vol. 9, 226-236, March 1968
Copyright © 1968 by Lipid Research, Inc.

Gas-liquid chromatographic determination of human fecal bile acids

Edmond Evrard and Gerard Janssen

Laboratory for Pathological Biochemistry of the St. Rafaëlsklinieken, and Rega Institute, Leuven, Belgium

A method for the determination of total bile acids in human feces that is suitable for routine application is described and discussed. Bile acids are extracted from freeze-dried feces with acetic acid and toluene, in the presence of the internal standard 23-nordeoxycholic acid. After saponification of the extract, bile acids and the internal standard are methylated and converted by mild chromic acid oxidation into their ketonic derivatives. The resultant mixture of a few stable compounds can be separated and measured quantitatively by gas-liquid chromatography on a methylsiloxane polymer. A reference bile acid mixture including the internal standard is also taken through the entire procedure with each series of samples. It has been demonstrated that, in spite of the omission of the usual purification steps, the method is specific for bile acids.

Supplementary key words fecal bile acids • man • extraction • hydrolysis • keto derivatives • gas-liquid chromatography • temperature programmed • reproducibility • antibiotics

Submitted on September 2, 1966
Revised on September 1, 1967
Accepted on December 4, 1967

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