Journal of Lipid Research, Vol. 9, 237-243, March 1968
Copyright © 1968 by Lipid Research, Inc.
Conversion of 7
,12
-dihydroxycholest-4-en-3-one to 5-cholestane-3,7,12-triol by iguana liver microsomes
T. Hoshita , S. Shefer , and E. H. Mosbach
Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York City Department of Health, New York 10009
The role of 7,12-dihydroxycholest-4-en-3-one as an intermediate in the formation of 5-bile acids from cholesterol was investigated with liver preparations of Iguana iguana in vitro. The microsomal fraction of iguana liver catalyzed the transformation of 7,12-dihydroxycholest-4-en-3-one to 5-cholestane-3,7,12-triol in good yield. 7,12-dihydroxy-5-cholestan-3-one served as an intermediate. Under the conditions employed, formation of the corresponding 5ßbeta;-isomers could not be detected. High speed supernatant solution and mitochondrial fraction of iguana liver did not reduce 7,12-dihydroxycholest-4-en-3-one to a measurable extent. The microsomal enzyme system required NADPH as hydrogen donor and was inactive in the presence of NADH.
It is suggested that 7,12-dihydroxycholest-4-en-3-one may serve as a common intermediate in the formation of 5- and 5ßbeta;-bile acids from cholesterol.
Supplementary key words Iguana iguana • allo bile acids • biosynthesis • liver microsomes • 7,12-dihydroxycholest-4-en-3-one • 7,12-dihydroxy-5-cholestan-3-one • 5-cholestane-3,7,12-triol • intermediary metabolism • bile acids
Submitted on October 18, 1967
Accepted on December 4, 1967
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