HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Weinhold, P. A. Search for Related Content PubMed PubMed Citation Articles by Weinhold, P. A. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 9, 262-266, March 1968
Copyright © 1968 by Lipid Research, Inc.

Biosynthesis of phosphatidyl choline during prenatal development of the rat lung

Paul A. Weinhold

Departments of Biological Chemistry and Internal Medicine (Nuclear Medicine), University of Michigan and Radioisotope Service, Veterans Administration Hospital, Ann Arbor, Michigan 48105

The biosynthesis of phosphatidyl choline in rat lung during prenatal development is of interest in connection with the mechanisms of functional development of the lung, particularly in relation to the production of lung surfactant and the control of phospholipid synthesis. The process was studied by determination of the ability of fetal and adult lung slices to incorporate choline-methyl-¹⁴C and methionine-methyl-¹⁴C into phosphatidyl choline.

The incorporation of radioactivity from methionine-methyl-¹⁴C was low in both adult and fetal lung. There was no apparent change in the incorporation during development. The incorporation of choline-methyl-¹⁴C into phosphatidyl choline after 60 min of incubation was low for fetal lung at 19 days gestation and increased to adult levels by 22 days gestation.

The incorporation of choline-methyl-¹⁴C into phosphoryl choline was high at 19 days gestation and decreased as development progressed. However, the specific activities of the phosphoryl choline were similar in fetal and adult lung, which suggests that the increase in incorporation of choline-methyl-¹⁴C into phosphatidyl choline with development results from an increased activity of reactions in the biosynthetic pathway between phosporyl choline and phosphatidyl choline.

Supplementary key words phosphatidyl choline • phosphoryl choline • biosynthesis • rat • lung • fetal development • choline-methyl-¹⁴C • methionine-methyl-¹⁴C

Submitted on August 11, 1967
Accepted on November 8, 1967

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?