J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 9, 285-286, March 1968
Copyright © 1968 by Lipid Research, Inc.

Analysis of fatty acids of human red cells without lipid extraction

Gerald B. Phillips , James T. Dodge , and Carole S. Rockmore

Department of Medicine, College of Physicians and Surgeons, Columbia University, and The Roosevelt Hospital, New York 10019

Exposure of human red cells to 2 n HCl for 18-20 hr at 110°C appears to release the total fatty acid, which can then be esterified for GLC analysis. This technique is simpler and may be more reliable than the conventional methods that depend on lipid extraction of the red cells.

Supplementary key words fatty acids • human red cells • hydrolysis • sphingomyelin • gas-liquid chromatography

Submitted on October 20, 1967
Accepted on December 5, 1967


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