J. Lipid Res.
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Journal of Lipid Research, Vol. 9, 288-290, March 1968
Copyright © 1968 by Lipid Research, Inc.

Alkaline solvent systems for thin-layer chromatography of bile acids

M. T. Subbiah and A. Kuksis

Department of Biochemistry, and Banting and Best Department of Medical Research, University of Toronto, Toronto 5, Canada

Thin-layer chromatographic separation of the common bile acids and their taurine and glycine conjugates in chloroform-methanol-ammonia is reported. An alkaline system offers advantages for the separation and nondestructive staining of bile acid conjugates.

Supplementary key words thin-layer chromatography • ammonia system • conjugated • nonconjugated • bile acids • nondestructive staining

Submitted on September 11, 1967
Accepted on December 4, 1967


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