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Journal of Lipid Research, Vol. 9, 371-373, May 1968
Copyright © 1968 by Lipid Research, Inc.

Enzymatic preparation and purification of prostaglandin E₂

Milton Lapidus , Norman H. Grant , and Harvey E. Alburn

Research Division, Wyeth Laboratories, Philadelphia, Pennsylvania 19101

An enzymatic system has been developed for the production of prostaglandin E₂ (PGE₂) from arachidonic acid by extracts of sheep seminal vesicular glands. The presence of glutathione insures high yields.

A new procedure for the purification of PGE₂ was also developed, based on the dialysis of the biosynthesized product at pH 8 and extraction of the dialysate at pH 3 with chloroform. This procedure routinely gives yields of PGE₂ of 25-37% (from arachidonic acid) with a purity of 90-100%.

Additional analytical proof of the identity of PGE₂ was provided by physicochemical characteristics of the crystalline thiosemicarbazide derivative, which can be readily prepared under mild conditions.

Supplementary key words prostaglandin • arachidonic acid • PGE₂ • enzymatic preparation • purification • dialysis • glutathione • PGE₂ thiosemicarbazone • IR spectra • thin-layer chromatography

Submitted on October 24, 1967
Accepted on February 13, 1968

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