Journal of Lipid Research, Vol. 9, 397-397, May 1968
Copyright © 1968 by Lipid Research, Inc.

Ferric chloride spray detector for cholesterol and cholesteryl esters on thin-layer chromatograms

Robert R. Lowry

Department of Agricultural Chemistry, Oregon State University, Corvallis, Oregon 97331

The use of a stable solution of ferric chloride for the detection of cholesterol and cholesteryl esters on thin-layer chromatographic layers is described. Its advantages over the commonly used Carr-Price reagent are listed, and the levels of sensitivity for the new reagent are shown. Detection of other lipid classes is also possible.

Supplementary key words thin-layer chromatography • cholesterol • cholesteryl esters • detection • ferric chloride spray • sensitivity levels

Submitted on September 21, 1967
Accepted on February 12, 1968

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