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Journal of Lipid Research, Vol. 9, 405-408, July 1968
Copyright © 1968 by Lipid Research, Inc.

7-Dehydrostigmasterol and ergosterol: the major sterols of an amoeba

Frank R. Smith and Edward D. Korn

Laboratory of Biochemistry, Section on Cellular Physiology, National Heart Institute, National Institutes of Health, Bethesda, Maryland 20014

The sterol fraction of Acanthamoeba (Neff) contains 60% 7-dehydrostigmasterol and 40% ergosterol. The sterols were characterized by infrared and ultraviolet spectroscopy, gas chromatography, specific reactions, and mass spectral analysis. Sterols constitute 5% of the lipids and 15% of the neutral lipids in this species.

Supplementary key words sterols • amoebae • Acanthamoeba (sp.) • ergosterol • 7-dehydrostigmasterol • taxonomy

Submitted on December 15, 1967
Accepted on February 26, 1968


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