J. Lipid Res.
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Journal of Lipid Research, Vol. 9, 409-415, July 1968
Copyright © 1968 by Lipid Research, Inc.

Lipid composition of chloroplasts isolated by aqueous and nonaqueous techniques

Alpaslan Ongun , W. W. Thomson , and J. B. Mudd

Department of Biochemistry, Department of Life Sciences, and Statewide Air Pollution Research Center, University of California, Riverside, California 92502

Chloroplasts isolated from tobacco leaves in 0.5 m sucrose solution (the 1000 g pellet) contained 83% of the total cellular monogalactosyl diglyceride, 88% of the digalactosyl diglyceride, 76% of the sulfolipid, and 74% of the phosphatidyl glycerol. Phosphatidyl inositol was concentrated in the 15,000 g pellet. Phosphatidyl choline and phosphatidyl ethanolamine were concentrated in the 15,000 g supernatant fraction.

Chloroplasts isolated from tobacco leaves by a nonaqueous technique in hexane-carbon tetrachloride show a glycerolipid composition similar to that found in chloroplasts isolated in the aqueous system, even though some lipid, particularly monogalactosyl diglyceride, is extracted by the organic solvent during the process.

Supplementary key words glycerolipids • distribution • cell fractions • tobacco leaves • chloroplast • nonaqueous isolation • electron microscopy • grana

Submitted on December 18, 1967
Accepted on March 5, 1968


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