Journal of Lipid Research, Vol. 9, 437-446, July 1968
Copyright © 1968 by Lipid Research, Inc.

Choline-deficiency fatty liver: impaired release of hepatic triglycerides

B. Lombardi , P. Pani , and F. F. Schlunk

Department of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15213

After intravenous injection of palmitate-1-¹⁴C to rats fed a choline-deficient (CD) or choline-supplemented (CS) diet for 15-18 hr, liver triglycerides became labeled very rapidly. In CS, but not in CD rats, there was a considerable loss, with time, of radioactivity from liver triglycerides. At the same time, significantly less radioactivity appeared in plasma triglycerides of CD rats than of CS animals. No difference was seen in the triglyceride content of microsomes isolated from the liver of rats fed the two diets. The lower radioactivity in plasma triglycerides of CD rats was essentially due to a lower level and specific activity of very low density lipoprotein triglycerides.

After intravenous injection of Triton and labeled palmitate, considerably less radioactivity accumulated in plasma triglycerides and phospholipids of CD rats than of CS animals. Post-Triton hyperphospholipidemia was also less pronounced in CD rats.

It was concluded that the fatty liver observed in CD rats results from an impaired release of hepatic triglycerides into plasma.

Supplementary key words choline deficiency • rat • pathogenesis • fatty liver • triglycerides • phospholipids • liver • microsomes • plasma • lipoproteins • chylomicra • intestine • palmitate incorporation • Triton • triglyceride secretion

Submitted on December 5, 1967
Accepted on March 11, 1968

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