Journal of Lipid Research, Vol. 9, 613-619, September 1968
Copyright © 1968 by Lipid Research, Inc.

Regulation of production and release of lipoprotein by the perfused rat liver

Neil B. Ruderman , Keith C. Richards , Victoria Valles De Bourges , and Albert L. Jones

Joslin Research Laboratory, Department of Medicine and Department of Anatomy, Harvard Medical School; and the Peter Bent Brigham Hospital and Diabetes Foundation, Inc., Boston, Massachusetts 02215

The relationship between protein and triglyceride release into d < 1.007 lipoprotein was studied in the isolated perfused rat liver. Livers were perfused with a medium either high or low in linoleate content. Perfusion with the linoleate-rich medium resulted in a marked increase in the net release of both d < 1.007 lipoprotein triglyceride and lipoprotein protein, and caused a significant increase in amino acid incorporation into the protein moiety. Amino acid incorporation into d 1.008-1.21 protein was not affected by fatty acid concentration, while incorporation into whole perfusate and tissue proteins was depressed by a perfusate high in fatty acid content. Electron microscopic studies demonstrated that the livers with the higher rate of triglyceride release also produced a greater number of lipoprotein particles. The particles they released were also somewhat larger.

These studies suggest that the intracellular concentration of newly esterified triglyceride and (or) some other lipid metabolite can specifically influence the release and perhaps the synthesis of d < 1.007 lipoprotein protein. They also suggest that the liver increases its rate of triglyceride release primarily by producing more lipoprotein particles.

Supplementary key words very low density lipoprotein • fatty acid esterification • triglyceride synthesis • protein synthesis • electron microscopy • lipoprotein particles • linoleate

Submitted on December 28, 1967
Accepted on May 29, 1968

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