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Journal of Lipid Research, Vol. 9, 620-626, September 1968
Copyright © 1968 by Lipid Research, Inc.

In vitro incorporation of cholesterol-¹⁴C into very low density lipoprotein cholesteryl esters

Yasuo Akanuma and John Glomset

Department of Medicine and Regional Primate Research Center, University of Washington, Seattle, Washington 98105

The cholesteryl esters of very low density lipoproteins become labeled when human plasma is incubated with cholesterol-¹⁴C. The relative order of magnitude of the specific activity of the cholesteryl esters of the major lipoprotein fractions is: high density lipoproteins >> very low density lipoproteins > low density lipoproteins. This pattern of labeling is similar to that found by others in experiments performed in vivo. Very low density lipoprotein cholesteryl esters are probably not formed by direct action of the plasma lecithin:cholesteryl acyltransferase, since significant esterification of cholesterol does not occur when very low density lipoproteins are incubated separately with the enzyme. Instead, labeled cholesteryl esters formed in the other lipoprotein fractions transfer to the very low density lipoproteins, the relative amount of monounsaturated esters transferred being slightly greater than that of saturated and polyunsaturated esters. The results support the possibility that the acyltransferase indirectly increases the concentration of very low density lipoprotein cholesteryl esters in vivo.

Supplementary key words lipoproteins • very low density • low density • high density • lecithin:cholesterol acyltransferase • cholesteryl esters • transfer • human plasma

Submitted on February 26, 1968
Accepted on May 24, 1968

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