Journal of Lipid Research, Vol. 9, 748-754, November 1968
Copyright © 1968 by Lipid Research, Inc.

Cytidine triphosphate: phosphatidic acid cytidyltransferase in Escherichia coli

James R. Carter Jr.

Diabetes Unit, Medical Services, Massachusetts General Hospital, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114

An enzyme has been found in particulate fractions of Escherichia coli that catalyzes the incorporation of cytidine triphosphate (CTP) into lipid in the presence of exogenous phosphatidic acid and Mg⁺⁺. The product has been identified enzymatically and by chromatography as cytidine diphosphate diglyceride. The reaction is optimal at a pH of 6.5 and Mg⁺⁺ concentration of 5-10 mm. The apparent K_m for CTP is 7 x 10^-4m and for phosphatidic acid, 2 x 10^-3m. The reaction rate falls off rapidly with time and ceases entirely after 1 hr as the result of inactivation of the system by Mg⁺⁺.

Supplementary key words CDP diglyceride • phospholipid biosynthesis • physical state of reaction mixture

Submitted on June 7, 1968
Accepted on July 29, 1968

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