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A more recent version of this article appeared on March 1, 2007 Originally published In Press as doi:10.1194/jlr.C600022-JLR200 on December 21, 2006

Papers In Press, published online ahead of print January 16, 2007
J. Lipid Res., doi:10.1194/jlr.C600022-JLR200
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Submitted on December 7, 2006
Revised on January 1, 1998
Accepted on December 20, 2006

Docosahexaenoic acid and arachidonic acid are fundamental supplements for induction of neuronal differentiation

Inna Kan, Eldad Melamed, Daniel Offen, and Pnina Green

Sackler School of Medicine, Tel Aviv University, Petah Tiqwa 49100

Corresponding Author: pgreen{at}post.tau.ac.il

Cell replacement therapy is being investigated for the treatment of neurodegenerative disorders. Adult autologous bone-marrow-derived mesenchymal stem cells (MSCs) have been induced to differentiate into neuron-like cells harboring a variety of neuronal markers and transcription factors. Neural tissue characteristically contains high proportions of docosahexaenoic acid (DHA) and arachidonic acid (AA). In the present study, evaluation of the fatty-acid profile of differentiated neuron-like cells revealed a very low level of DHA, similar to that in MSCs but different from typical neurons. Supplementation of the medium with DHA alone resulted in increased levels of DHA but concomitant low levels of AA. However, supplementation of both DHA and AA yielded a fatty acid profile resembling that of neural tissue. It also resulted in enhanced outgrowth of neurite-like processes, hallmarks of neuronal differentiation. These findings demonstrate the essentiality of DHA and AA supplementation in the process of induced neuronal differentiation and have important implications for the development of cell replacement strategies of neural repair.


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P. Kaur, K. Schulz, M. Aschner, and T. Syversen
Role of Docosahexaenoic Acid in Modulating Methylmercury-Induced Neurotoxicity
Toxicol. Sci., December 1, 2007; 100(2): 423 - 432.
[Abstract] [Full Text] [PDF]


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