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Papers In Press, published online ahead of print December 16, 2002
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Division of Clinical Preventive Medicine, Department of Community Preventive Medicine,, Niigata University Graduate School of Medical and Dental Sciences, Niigata City, Niigata 951-8510
Corresponding Author: miida{at}med.niigata-u.ac.jp
We have established an immunoassay for pre
Revised on November 29, 2002
Accepted on December 16, 2002
Analytical performance of a sandwich enzyme immunoassay for pre
1-HDL in stabilized plasma
1-HDL (the initial acceptor of cellular cholesterol) using a monoclonal antibody Mab55201. Because pre1-HDL is unstable during storage, fresh plasma must be used for pre1-HDL measurements. In this study, we describe a method of stabilizing pre1-HDL, and evaluate the analytical performance of the immunoassay for pre1-HDL. Fresh plasma was stored under various conditions with or without a pre-treatment consisting of a 21-fold dilution into 50% (v/v) sucrose. Pre1-HDL concentration was measured by the immunoassay. In non-pre-treated samples, pre1-HDL decreased significantly from the baseline after 6h at room temperature. Although pre1-HDL was more stable at 0ºC than at room temperature, it increased from 30.2±8.5 (SE) to 56.5±5.5 mg/L apoAI (p<0.001) in hyperlipimemics, and from 18.4±1.2 to 37.9±3.3 mg/L apoAI (p<0.001) in normolipidemics after 5-day storage. After 30-day storage at –80ºC, pre1-HDL increased from 29.0±4.0 to 38.0±5.7 mg/L apoAI (p<0.001) in hyperlipidemics, whereas it did not change in normolipidemics. In pre-treated samples, pre1-HDL concentration did not change significantly under any of the above conditions. Moreover, pre1-HDL concentrations determined by immunoassay correlated with those determined by native two-dimensional gel electrophoresis (n=24, r=0.833, p<0.05). An immunoassay using Mab55201 with pre-treated plasma is useful for clinical measurement of pre1-HDL.
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