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A more recent version of this article appeared on February 1, 2004

Papers In Press, published online ahead of print October 16, 2003
J. Lipid Res., doi:10.1194/jlr.D300024-JLR200
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Submitted on July 2, 2003
Revised on October 8, 2003
Accepted on October 14, 2003

A method of direct measurement for the enzymatic determination of cholesterol esters

Toshimi Mizoguchi, Toshiyuki Edano, and Tomoyuki Kosi

Tokyo Research Laboratories,, Pharmoceutical Division, Tokyo 189-0022

Corresponding Author: t-mizogt{at}kowa.co.jp

A direct measurement method for the enzymatic determination of cholesterol esters (CE) without measuring total cholesterol (TC) and free cholesterol (FC) are described. In the first step, hydrogen peroxide generated by cholesterol oxidase from FC was decomposed by catalase. In the second step, CE was measured by enzymatic determination using a colorimetric method or fluorometric method. The measurement sensitivity of fluorometric method was more than twenty times compared to colorimetric method. Optimal conditions of the assay were determined and examples of measured CE in human plasma, rat liver, and cultured cells were indicated. The method of directly measuring CE was simple and has exceptional reproducibility compared to the technique of subtracting FC from TC using each measured TC and F


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