J. Lipid Res.
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A more recent version of this article appeared on July 1, 2004

Papers In Press, published online ahead of print April 21, 2004
J. Lipid Res., doi:10.1194/jlr.D400001-JLR200
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Submitted on January 20, 2004
Revised on April 14, 2004
Accepted on April 18, 2004

In vivo MRS measurement of liver lipid levels in mice

Joel R. Garbow, Xiaobo Lin, Nobuhiro Sakata, Zhouji Chen, Dukho Koh, and Gustav Schonfeld

Biomedical MR Laboratory, Washington University, St. Louis, MO 63110

Corresponding Author: garbow{at}wustl.edu

A magnetic resonance spectroscopy (MRS) procedure for in vivo measurement of lipid levels in mouse liver is described and validated. The method uses respiratory-gated, localized spectroscopy to collect proton spectra from voxels within the mouse liver. Bayesian Probability Theory analysis of these spectra allows the relative intensities of the lipid and water resonances within the liver to be accurately measured. All spectral data were corrected for measured spin-spin (T2) relaxation. A total of 48 mice were used in this study, including wild-type mice and two different transgenics. Different groups of these mice were fed high-fat or low-fat diets or liquid diets, with and without the addition of alcohol. Proton spectra were collected at baseline and, subsequently, every 4 weeks for up to 16 weeks. Immediately following the last MRS measurement, mice were sacrificed and their livers analyzed for triglyceride level by conventional wet-chemistry methods. The excellent correlation between in vivo MRS and ex vivo wet-chemistry determinations of liver lipid validates the MRS method. The results clearly demonstrate that in vivo MRS will be an extremely valuable technique for longitudinal studies aimed at providing important insights into the genetic, environmental, and dietary factors affecting fat deposition and accumulation within the mouse liver.


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