J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on November 1, 2004

Papers In Press, published online ahead of print August 16, 2004
J. Lipid Res., doi:10.1194/jlr.D400010-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
D400010-JLR200v1
45/11/2145    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tanaka, T.
Right arrow Articles by Satouchi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanaka, T.
Right arrow Articles by Satouchi, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on June 14, 2004
Revised on August 3, 2004
Accepted on August 7, 2004

Quantitative analysis of lysophosphatidic acid by time-of-flight mass spectrometry using a phosphate capture molecule

Tamotsu Tanaka, Hideki Tsutsui, Kaoru Hirano, Tohru Koike, Akira Tokumura, and Kiyoshi Satouchi

Applied Biological Science, Fukuyama University, Fukuyama, Hiroshima 729-0292

Corresponding Author: tamot{at}fubac.fukuyama-u.ac.jp

Lysophosphatidic acid (LPA) is a lipid mediator that may play an important role in wound-healing, embryonic development and progression of cancer. Here, we report a procedure for the quantification of LPA by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The method is based on characteristic mass shift with total charge-change (from 2 to +1) of the phosphate species due to 1:1 complexation of LPA2- with a dinuclear zinc (II) complex (1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-2-olato dizinc(II) complex, Zn2L3+) at physiological pH. The monocationic complex [LPA2-- Zn2L3+]+ was detected in positive mode, where no other signal of cation adducts of LPA2- was observed. The detection limit of 18:1 LPA by this method was 0.1 pmol on sample plate. The intensity ratio of [LPA2-- Zn2L3+]+ against an internal standard [17:0 LPA2-- Zn2L3+]+ increased linearly with their molar ratio. Based on the relative intensities of complex ions, we determined amounts of LPA homologues in an egg white by this method, and found that results obtained were in good agreement with those by gas-liquid chromatography. This sensitive and convenient procedure for LPA-specific detection is useful for quantification of LPA homologues occurring in biological materials.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
E. Kinoshita, E. Kinoshita-Kikuta, K. Takiyama, and T. Koike
Phosphate-binding Tag, a New Tool to Visualize Phosphorylated Proteins
Mol. Cell. Proteomics, April 1, 2006; 5(4): 749 - 757.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.