J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 QUICK SEARCH:   [advanced]


     


A more recent version of this article appeared on February 1, 2005

Papers In Press, published online ahead of print November 1, 2004
J. Lipid Res., doi:10.1194/jlr.D400029-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
D400029-JLR200v1
46/2/373    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Owen, J. S.
Right arrow Articles by Thomas, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Owen, J. S.
Right arrow Articles by Thomas, M. J.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on October 13, 2004
Revised on November 1, 2004
Accepted on October 28, 2004

An improved assay for platelet-activating factor using HPLC-tandem mass spectrometry

John S. Owen, Robert L. Wykle, Michael P. Samuel, and Michael J. Thomas

Biochemistry Dept., Wake Forest University School of Medicine, Winston-Salem, NC 27157

Corresponding Author: joowen{at}wfubmc.edu

We describe an improved assay for platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) using HPLC-tandem mass spectrometry (LC-MS/MS). The present method can readily detect as little as 1 pg (1.9 fmol) of PAF, a significant improvement over previously described LC-MS/MS methods, and gives a linear response up to 1000 pg of PAF. Our method also overcomes the artifacts from isobaric lipids which have limited the usefulness of certain existing LC-MS/MS assays for PAF. In the course of these studies, we detected three novel lipid species in human neutrophils. One of the novel lipids appears to be a new molecular species of PAF, and the other two have chromatographic and mass spectrometric properties consistent with stearoyl-formyl-glycerophosphocholine and oleoyl-formyl-glycerophosphocholine. These observations identify previously unknown potential interferences in the measurement of PAF by LC-MS/MS. Moreover, our data suggest that the previously described palmitoyl-formyl-glycerophosphocholine is not unique, but rather is a member of a new and poorly understood family of formylated lipids.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
J. Lipid Res.Home page
K. Nakagawa, J.-H. Oak, O. Higuchi, T. Tsuzuki, S. Oikawa, H. Otani, M. Mune, H. Cai, and T. Miyazawa
Ion-trap tandem mass spectrometric analysis of Amadori-glycated phosphatidylethanolamine in human plasma with or without diabetes
J. Lipid Res., November 1, 2005; 46(11): 2514 - 2524.
[Abstract] [Full Text] [PDF]


Home page
J. Exp. Med.Home page
Y. Kihara, S. Ishii, Y. Kita, A. Toda, A. Shimada, and T. Shimizu
Dual phase regulation of experimental allergic encephalomyelitis by platelet-activating factor
J. Exp. Med., September 19, 2005; 202(6): 853 - 863.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.