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A more recent version of this article appeared on August 1, 2005

Papers In Press, published online ahead of print June 1, 2005
J. Lipid Res., doi:10.1194/jlr.D500014-JLR200
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Submitted on March 23, 2005
Revised on May 17, 2005
Accepted on May 18, 2005

Micro-extraction of bacterial lipid A: easy and rapid method for mass spectrometric characterization

Asmaa El hamidi, Alina Tirsoaga, Alexey Novikov, Ahmed Hussein, and Martine Caroff

Department of Biochemistry, Universite de Paris-Sud, ORSAY, Essone 91405

Corresponding Author: martine.caroff{at}bbmpc.u-psud.fr

Endotoxins (lipopolysaccharides, LPSs), are the main components of Gram-negative bacterial outer membranes. A quick and simple way to isolate their lipid region (lipid A) directly from whole bacterial cells was devised. This method using hot ammonium-isobutyrate solvent was applied to small quantities of cells and proved to be indispensable when a rapid characterization of lipid A structure by mass spectrometry was required. Biological activities of endotoxins are directly related to the lipid A structures which vary greatly with cell growth conditions. This method is suitable for rough and smooth-type bacteria and very efficient for screening variations in lipid A structures. Data are acquired in a few hours and avoid the use of phenol in extraction.


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This article has been cited by other articles:


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[Abstract] [Full Text] [PDF]


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