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Papers In Press, published online ahead of print June 16, 2005
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Advanced Medical Technology and Development, BML, Inc., Kawagoe, Saitama 350-1101
Corresponding Author: hhiro{at}bml.co.jp
Apolipoprotein (apo) A-V is a new member of the apolipoprotein gene family and has a role in triglyceride metabolism. We have developed an ELISA for apo A-V, in which the lower limit of detection is 0.3 ng/mL, with linearity up to 20 ng/mL, and have quantified plasma apo A-V concentration in healthy and diabetic subjects. In healthy subjects plasma total apo A-V concentration was 179.2 ± 74.8 ng/mL, and it was higher in females than in males (P<0.005). Plasma apo A-V concentration was correlated positively with high density lipoprotein (HDL) cholesterol (r=0.316, P<0.0001), apo A-I (r=0.269, P=0.0001) and apo E (r=0.180, P=0.011), and negatively with triglycerides (r=-0.218, P=0.021). In females but not males, apo A-V concentration was correlated positively with HDL cholesterol (r=0.277, P=0.0068), apo A-I (r=0.207, P=0.0435) and apo E (r=0.212, P=0.0388), and negatively with triglycerides (r=-0.228, P=0.0262). In the single nucleotide polymorphism of the SNP3 at the position -1131 nt of the apo A-V gene, apo A-V concentration was significantly higher in subjects with the T/T type than those with the C/C type (P<0.01), but not with the C/T type. In contrast, triglyceride concentration was significantly lower in subjects with the T/T type than in those with the C/C or C/T type (P<0.05). In addition, apo A-V concentration was much lower in non-insulin dependent diabetes mellitus than in healthy subjects (69.4 ± 44.3 ng/mL vs 179.2 ± 74.8 ng/mL, P<0.01). Our results show that plasma apo A-V concentration influences plasma triglyceride level, and that it is regulated by the polymorphism of the apo A-V gene. Plasma apo A-V concentrations are low in subjects with insulin resistance and diabetes mellitus.
Revised on May 31, 2005
Accepted on June 3, 2005
A sandwich enzyme-linked immunosorbent assay for human plasma apolipoprotein A-V concentration
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