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A more recent version of this article appeared on June 1, 2007

Papers In Press, published online ahead of print February 15, 2007
J. Lipid Res., doi:10.1194/jlr.D600042-JLR200
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Submitted on October 20, 2006
Revised on February 12, 2007
Accepted on February 15, 2007

Widespread occurrence of glyceryl ether monooxygenase activity in rat tissues detected by a novel assay

Ernst R. Werner, Albin Hermetter, Helmut Prast, Georg Golderer, and Gabriele Werner-Felmayer

Biocentre, Division of Biological Chemistry, Innsbruck Medical University, Innsbruck A-6020

Corresponding Author: ernst.r.werner{at}i-med.ac.at

An assay was set up for glyceryl ether monooxygenase activity in tissue samples using the novel substrate 1-O- pyrenedecyl-sn glycerol and high performance liquid chromatographic analysis of reaction mixtures with fluorescence detection, allowing robust detection of enzymatic activity in microgram amounts of tissue homogenates. The activity partially purified from rat liver strictly depended on presence of a tetrahydropteridine. Tetrahydrobiopterin-dependent glyceryl ether monooxygenase activity was observed in all rat tissues tested except female heart, with highest activities in liver, intestine and cerebellum. Activity was not uniformly distributed in brain, but was higher in cerebellum than in striatum or cortex. These data demonstrate that tetrahydrobiopterin-dependent glyceryl ether monooxygenase is not only found in liver and the gastrointestinal tract, but also in brain and other organs of the rat and provides an additional goal for tetrahydrobiopterin biosynthesis in these organs.


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