J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on May 1, 2007

Papers In Press, published online ahead of print February 4, 2007
J. Lipid Res., doi:10.1194/jlr.D700002-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
D700002-JLR200v1
48/5/1231    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fitzgerald, M.
Right arrow Articles by Murphy, R. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fitzgerald, M.
Right arrow Articles by Murphy, R. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on January 23, 2007
Revised on February 2, 2007
Accepted on February 3, 2007

Electrospray mass spectrometry of human hair wax esters

Mark Fitzgerald and Robert C. Murphy

Pharmacology, University of Colorado Health Sciences Center, Aurora, CO 80045-0511

Corresponding Author: robert.murphy{at}uchsc.edu

Wax esters extracted from human hair have been examined by capillary gas chromatography/mass spectrometry (GC/MS) and by nano electrospray ionization (ESI) mass spectrometry using a tandem quadrupole mass spectrometer. Initially, the wax esters were examined by capillary GC/MS using conventional means, thus revealing an incomplete chromatographic resolution of the complex array of more than 200 wax esters ranging from 28 - 40 carbons in length, including saturated/straight-chained, unsaturated/straight-chained, saturated/branched, and unsaturated/branched molecular species. Electrospray ionization of wax esters produced ammonium adduct ions [M+NH4]+, and collisional activation of these ions formed abundant [RCO2H2]+ product ions. Wax esters containing a double bond in the fatty acyl or fatty alcohol portion of the molecule revealed identical behavior suggesting little influence of the double bond on the ionization process or subsequent decomposition. The wax ester mixture was analyzed by ESI and tandem mass spectrometry using multiple reaction monitoring and neutral loss scanning. The neutral loss experiment (loss of NH3 and CH2=CH-(CH2)nCH3) was particularly effective in rapidly surveying the complex biological mixture, identifying more than 160 different wax esters that range from 24 - 42 total carbons.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
I. A. Butovich, E. Uchiyama, and J. P. McCulley
Lipids of human meibum: mass-spectrometric analysis and structural elucidation
J. Lipid Res., October 1, 2007; 48(10): 2220 - 2235.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.