J. Lipid Res. Please sign the JLR Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on January 1, 2003

Papers In Press, published online ahead of print October 16, 2002
J. Lipid Res., doi:10.1194/jlr.M200200-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M200200-JLR200v1
44/1/144    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spaniol, M.
Right arrow Articles by Krahenbuhl, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spaniol, M.
Right arrow Articles by Krahenbuhl, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on May 20, 2002
Revised on October 4, 2002
Accepted on October 10, 2002

Mechanisms of liver steatosis in rats with systemic carnitinedeficiency due to treatment with trimethylhydraziniumpropionate

Markus Spaniol, Priska Kaufmann, Konstantin Beier, Jenny Wuthrich, Michael Torok, Hubert Scharnagl, Winifried Marz, and Stephen Krahenbuhl

University Hospital, Basel CH-4031

Corresponding Author: kraehenbuehl{at}uhbs.ch

Summary Background: Rats with systemic carnitine deficiency induced by treatment with trimethylhydraziniumpropionate (THP) develop liver steatosis. Aims: To investigate the mechanisms leading to steatosis in THP-induced carnitine deficiency. Methods: Rats were treated with THP (20 mg/100 g) for 3 or 6 weeks, and were studied after starvation for 24 hours. Results: Rats treated with THP had reduced in vivo palmitate metabolism and developed mixed liver steatosis at both time points. The hepatic carnitine pool was reduced in THP-treated rats by 65 to 75% at both time points. Liver mitochondria from THP-treated rats had increased oxidative metabolism of various substrates and of â -oxidation at 3 weeks, but reduced activities at 6 weeks of THP treatment. Ketogenesis was not affected. The hepatic content of coenzyme A was increased by 23% at 3 and by 40% at 6 weeks in THP treated rats. The cytosolic content of long-chain acyl-CoAs was increased and the mitochondrial content decreased in hepatocytes of THP treated rats, compatible with decreased activity of carnitine palmitoyltransferase I in vivo. THP-treated rats showed hepatic peroxisomal proliferation and increased plasma VLDL triglyceride and phospholipid concentrations at both time points. Conclusions: A reduction in the hepatic carnitine pool is the principle mechanism leading to impaired hepatic fatty acid metabolism and liver steatosis in THP-treated rats. Cytosolic accumulation of long-chain acyl-CoAs is associated with increased plasma VLDL triglyceride and phospholipid concentrations and peroxisomal proliferation. Key words: systemic carnitine deficiency, liver steatosis, mitochondrial and peroxisomal beta-oxidation, peroxisomal proliferation, trimethylhydraziniumpropionate


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Pharmacol. Exp. Ther.Home page
A. C. Knapp, L. Todesco, K. Beier, L. Terracciano, H. Sagesser, J. Reichen, and S. Krahenbuhl
Toxicity of Valproic Acid in Mice with Decreased Plasma and Tissue Carnitine Stores
J. Pharmacol. Exp. Ther., February 1, 2008; 324(2): 568 - 575.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Degrace, L. Demizieux, Z.-y. Du, J. Gresti, L. Caverot, L. Djaouti, T. Jourdan, B. Moindrot, J.-C. Guilland, J.-F. Hocquette, et al.
Regulation of Lipid Flux between Liver and Adipose Tissue during Transient Hepatic Steatosis in Carnitine-depleted Rats
J. Biol. Chem., July 20, 2007; 282(29): 20816 - 20826.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
D. B. Carlson, J. W. McFadden, A. D'Angelo, J. C. Woodworth, and J. K. Drackley
Dietary L-Carnitine Affects Periparturient Nutrient Metabolism and Lactation in Multiparous Cows
J Dairy Sci, July 1, 2007; 90(7): 3422 - 3441.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
P. D. Cornwell and R. G. Ulrich
Investigating the Mechanistic Basis for Hepatic Toxicity Induced by an Experimental Chemokine Receptor 5 (CCR5) Antagonist Using a Compendium of Gene Expression Profiles
Toxicol Pathol, June 1, 2007; 35(4): 576 - 588.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
D. B. Carlson, N. B. Litherland, H. M. Dann, J. C. Woodworth, and J. K. Drackley
Metabolic Effects of Abomasal L-Carnitine Infusion and Feed Restriction in Lactating Holstein Cows
J Dairy Sci, December 1, 2006; 89(12): 4819 - 4834.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
S. G. Correa, M. C. Rodriguez-Galan, B. Salido-Renteria, R. Cano, H. Cejas, and C. E. Sotomayor
High dissemination and hepatotoxicity in rats infected with Candida albicans after stress exposure: potential sensitization to liver damage
Int. Immunol., December 1, 2004; 16(12): 1761 - 1768.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.