| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print September 16, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 15, 2002
Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1554
Corresponding Author: ntambi{at}biochem.wisc.edu
The mouse preputial gland (PG), a specialized sebaceous structure, is rich in wax esters, triglycerides and alkyl-2,3-diacylglycerol. We have found that the mouse PG expresses the three gene isoforms (SCD1, SCD2 and SCD3) of the D9 stearoyl-CoA desaturase enzyme that catalyzes the biosynthesis of monounsaturated fatty acids mainly, C16:1n-7 and C18:1n-9. However, mice with a targeted disruption in the SCD1 isoform (SCD1-/-) have undetectable SCD3 mRNA expression in the PG while the expression of SCD2 isoform was not altered. The levels of C16:1n-7 were reduced by greater than 70% while that of C18:1n-9 were reduced by 28%. The content of the C16:1n-10 (D6 hexadecenoic acid) isomer and a major fatty acid of the PG was increased by greater than 2-fold mainly in the wax ester fraction of the SCD1-/- mouse. We demonstrate that the increase in C16:1n-10 is due to induction of a specific palmitoyl-CoA D6 desaturase activity. Testosterone administration to the SCD1-/- mouse induced SCD3 mRNA expression and resulted in an increase in the D9 desaturation of 16:0-CoA but not of 18:0-CoA. These observations demonstrate that loss of SCD1 function alters the expression of SCD3 and reveal for the first time the presence and regulation of a palmitoyl-CoA D6 desaturase enzyme in mammals
Revised on September 16, 2002
Accepted on September 5, 2002
Lack of stearoyl-CoA desaturase-1 function induces a palmitoyl-CoA D6 desaturase and represses the stearoyl-CoA desaturase-3 gene in the preputial glands of the mouse
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Momozawa, Y. Takeuchi, M. Kitago, K. Masuda, Y. Kakuma, C. Hashizume, T. Ichimaru, K. Mogi, H. Okamura, T. Yonezawa, et al. Gene Expression Profiles Linked to the Hormonal Induction of Male-Effect Pheromone Synthesis in Goats (Capra hircus) Biol Reprod, July 1, 2007; 77(1): 102 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miyazaki, S. M. Bruggink, and J. M. Ntambi Identification of mouse palmitoyl-coenzyme A {Delta}9-desaturase J. Lipid Res., April 1, 2006; 47(4): 700 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miyazaki, A. Dobrzyn, P. M. Elias, and J. M. Ntambi Stearoyl-CoA desaturase-2 gene expression is required for lipid synthesis during early skin and liver development PNAS, August 30, 2005; 102(35): 12501 - 12506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Cheng and D. W. Russell Mammalian Wax Biosynthesis: II. EXPRESSION CLONING OF WAX SYNTHASE cDNAs ENCODING A MEMBER OF THE ACYLTRANSFERASE ENZYME FAMILY J. Biol. Chem., September 3, 2004; 279(36): 37798 - 37807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Rahman, A. Dobrzyn, P. Dobrzyn, S.-H. Lee, M. Miyazaki, and J. M. Ntambi Stearoyl-CoA desaturase 1 deficiency elevates insulin-signaling components and down-regulates protein-tyrosine phosphatase 1B in muscle PNAS, September 16, 2003; 100(19): 11110 - 11115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyazaki, M. J. Jacobson, W. C. Man, P. Cohen, E. Asilmaz, J. M. Friedman, and J. M. Ntambi Identification and Characterization of Murine SCD4, a Novel Heart-specific Stearoyl-CoA Desaturase Isoform Regulated by Leptin and Dietary Factors J. Biol. Chem., September 5, 2003; 278(36): 33904 - 33911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Guillou, V. Rioux, D. Catheline, J.-N. Thibault, M. Bouriel, S. Jan, S. D'Andrea, and P. Legrand Conversion of hexadecanoic acid to hexadecenoic acid by rat {Delta}6-desaturase J. Lipid Res., March 1, 2003; 44(3): 450 - 454. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
