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J. Lipid Res.
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A more recent version of this article appeared on December 1, 2002

Papers In Press, published online ahead of print September 16, 2002
J. Lipid Res., doi:10.1194/jlr.M200305-JLR200
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Submitted on August 5, 2002
Revised on September 10, 2002
Accepted on September 10, 2002

Nitric oxide promotes differentiation of rat white preadipocytes in culture

Hongyun Yan, Edith Aziz, Gillian Shillabeer, Alex Wong, Deena Shanghavi, Abdenaim Kermouni, Mohammed Abdel-Hafez, and David C. W. Lau

Medicine Dept., Julia-McFarlane Diabetes Center, Health Science Center,The University of Calgary, Calgary, AB T2N 4N1

Corresponding Author: dcwlau{at}ucalgary.ca

The putative role of nitric oxide (NO) in modulating adipogenesis was investigated in cultured preadipocytes derived from rat white adipose tissue. The NO releasing reagent, hydroxylamine (HA), and nitric oxide synthase (NOS) substrate, L-arginine (Arg), had no influence on cell replication. However, both HA and Arg exhibited significant induction on differentiation, as evidenced by increased lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (GPDH) activities, as well as accelerated triacylglycerol (TG) accumulation. These observations suggested a positive role of NO in modulating adipogenesis. Preadipocytes were found to produce NO, and a ~50% increase over basal level was observed on the first 2 days of differentiation. Deprivation of endogenous NOS activity by a non-selective NOS inhibitor, NG-monomethyl-L-arginine (NMMA), partially abrogated the differentiation process, implicating a role for endogenous NO to stimulate preadipocyte differentiation. Both NOS isoforms, eNOS and iNOS, were detected in differentiating preadipocytes. Specific iNOS inhibitors (1400W and aminoguanidine) had little influence on NO production and differentiation, suggesting that eNOS rather than iNOS may be the major isoform involved in modulating adipogenesis.


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