J. Lipid Res.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 1, 2003

Papers In Press, published online ahead of print April 1, 2003
J. Lipid Res., doi:10.1194/jlr.M200429-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M200429-JLR200v1
44/6/1132    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thuahnai, S. T.
Right arrow Articles by Phillips, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thuahnai, S. T.
Right arrow Articles by Phillips, M. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on November 4, 2002
Revised on March 24, 2003
Accepted on March 31, 2003

A quantitative analysis of apolipoprotein binding to scavenger receptor class B, type I (SR-BI): Multiple binding sites for lipid-free and lipid-associated apolipoproteins

Stephen T. Thuahnai, Sissel Lund-Katz, G.M. Anantharamaiah, David L. Williams, and Michael C. Phillips

GI/Nutrition, Children's Hospital of Philadelphia, Philadelphia, PA 19104-4318

Corresponding Author: phillipsmi{at}email.chop.edu

Competitive binding experiments were performed using Y1-BS1 adrenal cells to provide information about the interaction of HDL apolipoproteins (apo) with scavenger receptor class B, type 1 (SR-BI). Exchangeable apo A-I, A-II, E-2, E-3 and E-4 as phospholipid complexes bind like HDL3 to SR-BI via their multiple amphipathic alpha-helices; the concentrations required to reduce the binding of HDL3 to SR-BI by 50% (IC50) were similar and in the range 35-50 mg protein/ml. In the case of apo A-I, peptides corresponding to segments 1-85, 44-65, 44-87, 149-243 and 209-241 all had the same IC50 as each other (p=0.86) showing that a specific amino acid sequence in apo A-I is not responsible for the interaction with SR-BI. The distribution of charged residues in the amphipathic alpha-helix affects the interaction, with class A and Y helices binding better than class G* helices. Synthetic alpha-helical peptides composed of either L or D amino acids can bind equally to the receptor. Association with phospholipid increases the amount of apo binding to SR-BI without altering the affinity of binding. Lipid-free apo compete only partially with the binding of HDL to SR-BI whereas lipidated apo compete fully. These results are consistent with the existence of more than one type of apo binding site on SR-BI.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
M. Van Eck, M. Hoekstra, R. Out, I. S. T. Bos, J. K. Kruijt, R. B. Hildebrand, and T. J. C. Van Berkel
Scavenger receptor BI facilitates the metabolism of VLDL lipoproteins in vivo
J. Lipid Res., January 1, 2008; 49(1): 136 - 146.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Kurumada, A. Onishi, H. Imai, K. Ishii, T. Kobayashi, and S. B. Sato
Stage-Specific Association of Apolipoprotein A-I and E in Developing Mouse Retina
Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1815 - 1823.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
A. H.E.M. Klerkx, K. E. Harchaoui, W. A. van der Steeg, S. M. Boekholdt, E. S.G. Stroes, J. J.P. Kastelein, and J. A. Kuivenhoven
Cholesteryl Ester Transfer Protein (CETP) Inhibition Beyond Raising High-Density Lipoprotein Cholesterol Levels: Pathways by Which Modulation of CETP Activity May Alter Atherogenesis
Arterioscler. Thromb. Vasc. Biol., April 1, 2006; 26(4): 706 - 715.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. A. Ajees, G. M. Anantharamaiah, V. K. Mishra, M. M. Hussain, and H. M. K. Murthy
Crystal structure of human apolipoprotein A-I: Insights into its protective effect against cardiovascular diseases
PNAS, February 14, 2006; 103(7): 2126 - 2131.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
B. F. Asztalos, M. de la Llera-Moya, G. E. Dallal, K. V. Horvath, E. J. Schaefer, and G. H. Rothblat
Differential effects of HDL subpopulations on cellular ABCA1- and SR-BI-mediated cholesterol efflux
J. Lipid Res., October 1, 2005; 46(10): 2246 - 2253.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
N. Dashti, G. Datta, M. Manchekar, M. Chaddha, and G. M. Anantharamaiah
Model class A and class L peptides increase the production of apoA-I-containing lipoproteins in HepG2 cells
J. Lipid Res., October 1, 2004; 45(10): 1919 - 1928.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
B. Zhang, P. Fan, E. Shimoji, H. Xu, K. Takeuchi, C. Bian, and K. Saku
Inhibition of Cholesteryl Ester Transfer Protein Activity by JTT-705 Increases Apolipoprotein E-Containing High-Density Lipoprotein and Favorably Affects the Function and Enzyme Composition of High-Density Lipoprotein in Rabbits
Arterioscler. Thromb. Vasc. Biol., October 1, 2004; 24(10): 1910 - 1915.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Rhainds, P. Bourgeois, G. Bourret, K. Huard, L. Falstrault, and L. Brissette
Localization and regulation of SR-BI in membrane rafts of HepG2 cells
J. Cell Sci., July 1, 2004; 117(15): 3095 - 3105.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Parathath, D. Sahoo, Y. F. Darlington, Y. Peng, H. L. Collins, G. H. Rothblat, D. L. Williams, and M. A. Connelly
Glycine 420 Near the C-terminal Transmembrane Domain of SR-BI Is Critical for Proper Delivery and Metabolism of High Density Lipoprotein Cholesteryl Ester
J. Biol. Chem., June 11, 2004; 279(24): 24976 - 24985.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
L. Krimbou, M. Denis, B. Haidar, M. Carrier, M. Marcil, and J. Genest Jr.
Molecular interactions between apoE and ABCA1: impact on apoE lipidation
J. Lipid Res., May 1, 2004; 45(5): 839 - 848.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
M. C. de Beer, L. W. Castellani, L. Cai, A. J. Stromberg, F. C. de Beer, and D. R. van der Westhuyzen
ApoA-II modulates the association of HDL with class B scavenger receptors SR-BI and CD36
J. Lipid Res., April 1, 2004; 45(4): 706 - 715.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. T. Thuahnai, S. Lund-Katz, P. Dhanasekaran, M. de la Llera-Moya, M. A. Connelly, D. L. Williams, G. H. Rothblat, and M. C. Phillips
Scavenger Receptor Class B Type I-mediated Cholesteryl Ester-selective Uptake and Efflux of Unesterified Cholesterol: INFLUENCE OF HIGH DENSITY LIPOPROTEIN SIZE AND STRUCTURE
J. Biol. Chem., March 26, 2004; 279(13): 12448 - 12455.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
D. Wustner, M. Mondal, A. Huang, and F. R. Maxfield
Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells
J. Lipid Res., March 1, 2004; 45(3): 427 - 437.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
N. R. Webb, M. C. de Beer, F. C. de Beer, and D. R. van der Westhuyzen
ApoB-containing lipoproteins in apoE-deficient mice are not metabolized by the class B scavenger receptor BI
J. Lipid Res., February 1, 2004; 45(2): 272 - 280.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
B. L. Trigatti, M. Krieger, and A. Rigotti
Influence of the HDL Receptor SR-BI on Lipoprotein Metabolism and Atherosclerosis
Arterioscler. Thromb. Vasc. Biol., October 1, 2003; 23(10): 1732 - 1738.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.