| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print January 16, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, NY 11794-5215
Corresponding Author: deborah.brown{at}sunysb.edu
Free cholesterol (FC) has been reported to efflux from cells through caveolae, which are 50-100 nm plasma membrane pits. The 22 kDa protein caveolin-1 is concentrated in caveolae and is required for their formation. The HDL receptor SR-BI, which stimulates both FC efflux and selective uptake of HDL-derived cholesteryl ester (CE), has been reported to be concentrated in caveolae, suggesting that this localization facilitates flux of FC and CE across the membrane. However, we found that overexpression of caveolin-1 in Fischer rat thyroid (FRT) cells, which lack caveolin-1 and caveolae, or HEK 293 cells, which normally express very low levels of caveolin-1, did not affect FC efflux to HDL or liposomes. Transient expression of SR-B1 did not affect this result. Similarly, caveolin-1 expression did not affect selective uptake of CE from labeled HDL particles in FRT or HEK 293 cells transfected with SR-BI. We conclude that basal and SR-BI-stimulated FC efflux to HDL and liposomes, and SR-BI-mediated selective uptake of HDL CE, are not affected by caveolin-1 expression in HEK 293 or FRT cells.
Revised on January 14, 2003
Accepted on January 14, 2003
Caveolin-1 does not affect SR-BI-mediated cholesterol efflux or selective uptake of cholesteryl ester in two cell lines
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. G. Frank, S. Pavlides, M. W.-C. Cheung, K. Daumer, and M. P. Lisanti Role of caveolin-1 in the regulation of lipoprotein metabolism Am J Physiol Cell Physiol, July 1, 2008; 295(1): C242 - C248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Sengupta, F. Philip, and S. Scarlata Caveolin-1 alters Ca2+ signal duration through specific interaction with the G{alpha}q family of G proteins J. Cell Sci., May 1, 2008; 121(9): 1363 - 1372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-K. Cha, T. Wu, and C.-L. Huang Protein kinase C inhibits caveolae-mediated endocytosis of TRPV5 Am J Physiol Renal Physiol, May 1, 2008; 294(5): F1212 - F1221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Lin, C. Ma, W.-C. Hsu, H.-F. Lo, and V. C. Yang Molecular interaction between caveolin-1 and ABCA1 on high-density lipoprotein-mediated cholesterol efflux in aortic endothelial cells Cardiovasc Res, August 1, 2007; 75(3): 575 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wang, H. Mu, H. Chai, D. Liao, Q. Yao, and C. Chen Human Immunodeficiency Virus Protease Inhibitor Ritonavir Inhibits Cholesterol Efflux from Human Macrophage-Derived Foam Cells Am. J. Pathol., July 1, 2007; 171(1): 304 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Frank, M. W.-C. Cheung, S. Pavlides, G. Llaverias, D. S. Park, and M. P. Lisanti Caveolin-1 and regulation of cellular cholesterol homeostasis Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H677 - H686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Harder, G. Vassiliou, H. M. McBride, and R. McPherson Hepatic SR-BI-mediated cholesteryl ester selective uptake occurs with unaltered efficiency in the absence of cellular energy J. Lipid Res., March 1, 2006; 47(3): 492 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wharton, T. Meshulamy, G. Vallega, and P. Pilch Dissociation of Insulin Receptor Expression and Signaling from Caveolin-1 Expression J. Biol. Chem., April 8, 2005; 280(14): 13483 - 13486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fu, A. Hoang, G. Escher, R. G. Parton, Z. Krozowski, and D. Sviridov Expression of Caveolin-1 Enhances Cholesterol Efflux in Hepatic Cells J. Biol. Chem., April 2, 2004; 279(14): 14140 - 14146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Levy, D. Menard, I. Suc, E. Delvin, V. Marcil, L. Brissette, L. Thibault, and M. Bendayan Ontogeny, immunolocalisation, distribution and function of SR-BI in the human intestine J. Cell Sci., January 15, 2004; 117(2): 327 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Peng, W. Akmentin, M. A. Connelly, S. Lund-Katz, M. C. Phillips, and D. L. Williams Scavenger Receptor BI (SR-BI) Clustered on Microvillar Extensions Suggests that This Plasma Membrane Domain Is a Way Station for Cholesterol Trafficking between Cells and High-Density Lipoprotein Mol. Biol. Cell, January 1, 2004; 15(1): 384 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Schmid, W. S. Davidson, L. Myatt, and L. A. Woollett Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulation J. Lipid Res., October 1, 2003; 44(10): 1909 - 1918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Trigatti, M. Krieger, and A. Rigotti Influence of the HDL Receptor SR-BI on Lipoprotein Metabolism and Atherosclerosis Arterioscler. Thromb. Vasc. Biol., October 1, 2003; 23(10): 1732 - 1738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rigotti, H. E. Miettinen, and M. Krieger The Role of the High-Density Lipoprotein Receptor SR-BI in the Lipid Metabolism of Endocrine and Other Tissues Endocr. Rev., June 1, 2003; 24(3): 357 - 387. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
