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A more recent version of this article appeared on October 1, 2003

Papers In Press, published online ahead of print August 1, 2003
J. Lipid Res., doi:10.1194/jlr.M300126-JLR200
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Submitted on March 25, 2003
Revised on June 24, 2003
Accepted on July 17, 2003

The transport of cholesterol across a BeWo cell monolayer: Implications for net transport of sterol from the maternal to fetal circulation

Kara E. Schmid, W. Sean Davidson, Leslie Myatt, and Laura A. Woollett

Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267

Corresponding Author: laura.woollett{at}uc.edu

The placental transport of various compounds, such as glucose and fatty acids, has been well studied. However the transport of cholesterol, a sterol essential for proper fetal development, remains undefined in the placenta. Therefore, the purpose of these studies was to examine the transport of cholesterol across a placental monolayer and its uptake by various cholesterol acceptors. BeWo cells, which originated from a human choriocarcinoma, were grown on transwells for 3 days to form a confluent monolayer. The apical side of the cells was radiolabeled with either free cholesterol or LDL cholesteryl ester. After 24 hours the radiolabel was removed and cholesterol acceptors were added to the basolateral chamber. Cholesterol was found to be taken up by the apical surface of the placental monolayer, transported to the basolateral surface of the cell and effluxed to either fetal human serum, fetal HDL or phospholipid vesicles, but not to apolipoprotein A-I. In addition, increasing the cellular cholesterol concentration further increased the amount of cholesterol transported to the basolateral acceptors. These are the first studies to demonstrate the movement of cholesterol across a placental cell from the maternal circulation (apical side) to the fetal circulation (basolateral side).


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