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Papers In Press, published online ahead of print February 16, 2004
J. Lipid Res., doi:10.1194/jlr.M300435-JLR200
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UMR 585 Inserm/Insa, Villeurbanne 69100
Corresponding Author: valerie.pruneta-deloche{at}insa-lyon.fr
Postprandial hyperlipidemia is considered to be proatherogenic in type 2 diabetes. An important determinant of postprandial lipid metabolism is the activity of lipoprotein lipase (LPL) which is responsible for hydrolysis of triglycerides. The time-course of LPL activity in the postprandial state following one single meal has never been studied since the determination of lipoprotein lipase activity requires heparin injection. Recently, we have shown that LPL activity could be accurately measured in non-heparinized VLDL using a new assay that we developed to determine the LPL-dependent VLDL-triglyceride (TG) hydrolysis (LVTH). Based on the same principle, we used in the present study a TG-rich lipoprotein (TRL)-bound LPL-dependent TRL-TG hydrolysis (LTTH) to compare the time-course of LPL activity of 10 patients with type 2 diabetes to that of 10 healthy subjects, following the ingestion of a lipid-rich test meal. In both groups, the peak triglyceride concentration was reached after 4 h and was 67% higher in type 2 diabetics (P < 0.005). Fasting LTTH were 91.3 +/- 15.6 in controls vs 70.1 +/- 4.8 nmol NEFA/ml.h in type 2 diabetics (P < 0.001). LTTH was increased 2 h postprandially by 190% in controls and by only 89% in diabetic patients, finally resulting in a 35% lowering of LTTH area under the curve in diabetics as compared to controls. Postprandial LTTH was inversely correlated to either triglyceride or remnant concentrations in controls but not in diabetics, while LTTH was inversely correlated to insulin resistance in both groups. These data provide evidence that TRL-bound LPL activity increases in human subjects in the postprandial state and is strongly reduced in type 2 diabetes, thereby contributing to the observed postprandial hypertriglyceridemia.
Revised on February 2, 2004
Accepted on February 10, 2004
Alteration in lipoprotein lipase activity bound to triglyceride-rich lipoproteins in the postprandial state in type 2 diabetes
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