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A more recent version of this article appeared on August 1, 2004

Papers In Press, published online ahead of print May 16, 2004
J. Lipid Res., doi:10.1194/jlr.M400003-JLR200
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Submitted on January 6, 2004
Revised on May 10, 2004
Accepted on May 13, 2004

Subcellular localization of the mosquito sterol carrier protein-2 and sterol carrier protein-x

Que Lan and Randall J. Massey

Entomology, University of Wisconsin-Madison, Madison, WI 53706

Corresponding Author: qlan{at}entomology.wisc.edu

Subcellular distribution of Aedes aegypti (Ae. aegypti) sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-x (AeSCP-x) was studied using electron microscopy. In both cultured Ae. aegypti cells and in the larval midgut, AeSCP-2 was detected mostly in the cytosol, with some labeling mitochondria and nucleus, but, not in membranous vesicles. The widespread distribution of AeSCP-2 in the midgut epithelium is consistent with its potential lipid transfer function in all phases of cholesterol absorption. In contrast, AeSCP-x was found mostly in the peroxisome. Differences in the subcellular distribution of AeSCP-2 and AeSCP-x suggest that these two members of the SCP-2 gene family are functionally distinct. Over-expression of AeSCP-2 in Aedes aegypti cells showed increased localization of AeSCP-2 to cytosol, mitochondria and nucleus. This is the first report on the nuclear distribution of a sterol carrier protein. Over-expression of AeSCP-2 resulted in increased cholesterol incorporation in cells suggesting AeSCP-2 enhances cholesterol uptake.


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