| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
Papers In Press, published online ahead of print February 1, 2005
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Medicine, University of California, San Diego, La Jolla, CA 92093
Corresponding Author: oquehenberger{at}ucsd.edu
We have previously shown that CD36 recognizes oxidation products of phospholipids on oxidized LDL (OxLDL) such as 1-palmitoyl-2-(5’-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC). The current study was designed to examine whether the phosphocholine (PC) headgroup in POVPC constitutes an obligatory binding target for CD36. To examine the contribution of PC in the binding of POVPC to CD36, we used well-defined synthetic oxidized phospholipids (OxPL) crosslinked to BSA or to a hexapeptide. The OxPL-adducts were then tested for their ability to bind to CD36-transfected cells and for their ability to inhibit OxLDL binding to CD36. Both POVPC-BSA and POVPC-peptide adducts were high affinity ligands for CD36 and potent inhibitors of OxLDL binding. Enzymatic removal of the entire PC moiety of the POVPC-peptide, or of the choline headgroup alone, as well as substitution of the choline headgroup by ethanolamine abrogated the inhibitory activity of POVPC. Interestingly, PC by itself or crosslinked to BSA did not show any intrinsic competition activity. In conclusion, our data demonstrate that the PC headgroup of OxPL alone is sufficient for binding to CD36, but only if presented in the correct conformation as in OxPL of OxLDL or as in POVPC-peptide adducts.
Revised on January 27, 2005
Accepted on January 31, 2005
Phosphocholine as a pattern recognition ligand for CD36
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Harkewicz, K. Hartvigsen, F. Almazan, E. A. Dennis, J. L. Witztum, and Y. I. Miller Cholesteryl Ester Hydroperoxides Are Biologically Active Components of Minimally Oxidized Low Density Lipoprotein J. Biol. Chem., April 18, 2008; 283(16): 10241 - 10251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. G. Gugiu, K. Mouillesseaux, V. Duong, T. Herzog, A. Hekimian, L. Koroniak, T. M. Vondriska, and A. D. Watson Protein targets of oxidized phospholipids in endothelial cells J. Lipid Res., March 1, 2008; 49(3): 510 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Greenberg, X.-M. Li, B. G. Gugiu, X. Gu, J. Qin, R. G. Salomon, and S. L. Hazen The Lipid Whisker Model of the Structure of Oxidized Cell Membranes J. Biol. Chem., January 25, 2008; 283(4): 2385 - 2396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-J. Won, M. F. Bachmann, and J. F. Kearney CD36 Is Differentially Expressed on B Cell Subsets during Development and in Responses to Antigen J. Immunol., January 1, 2008; 180(1): 230 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Harkewicz, E. Fahy, A. Andreyev, and E. A. Dennis Arachidonate-derived Dihomoprostaglandin Production Observed in Endotoxin-stimulated Macrophage-like Cells J. Biol. Chem., February 2, 2007; 282(5): 2899 - 2910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Mwaikambo, F. Sennlaub, H. Ong, S. Chemtob, and P. Hardy Activation of CD36 Inhibits and Induces Regression of Inflammatory Corneal Neovascularization. Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4356 - 4364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Binder, P. X. Shaw, M.-K. Chang, A. Boullier, K. Hartvigsen, S. Horkko, Y. I. Miller, D. A. Woelkers, M. Corr, and J. L. Witztum Thematic review series: The Immune System and Atherogenesis. The role of natural antibodies in atherogenesis J. Lipid Res., July 1, 2005; 46(7): 1353 - 1363. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
