J. Lipid Res. Did you know there is a large type edition? Click here.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on July 1, 2005

Papers In Press, published online ahead of print April 16, 2005
J. Lipid Res., doi:10.1194/jlr.M500058-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M500058-JLR200v1
46/7/1388    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Touboul, D.
Right arrow Articles by Laprévote, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Touboul, D.
Right arrow Articles by Laprévote, O.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on February 16, 2005
Revised on April 5, 2005
Accepted on April 8, 2005

Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: application to Duchenne muscular dystrophy

David Touboul, Alain Brunelle, Frédéric Halgand, Sabine De La Porte, and Olivier Laprévote

ICSN, CNRS, Gif-sur-Yvette, France 91198

Corresponding Author: Alain.Brunelle{at}icsn.cnrs-gif.fr

Imaging with time-of-flight secondary ion mass spectrometry (TOF-SIMS) has expanded very rapidly with the development of gold cluster ion sources (Au3+). It is now possible to acquire ion density maps (ion images) on a tissue section, without any treatment and with a lateral resolution of few micrometers. In this article, we have taken advantage of this technique to study the degeneration/regeneration process in muscles of a Duchenne Muscular Dystrophy model mouse. Specific distribution of different lipid classes (fatty acids, triglycerids, phospholipids, tocopherol, coenzyme Q9 and cholesterol) allows us to distinguish three different regions on a mouse leg section: one is destructured, while another one is in degeneration (oxidative stress and deregulation of the phosphoinositol cycle) and the last one is stable. TOF SIMS imaging shows here great capabilities to localize directly on a tissue section, a great number of lipid compounds, which reflect the state of the cellular metabolism.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Lipid Res.Home page
N. Tahallah, A. Brunelle, S. De La Porte, and O. Laprevote
Lipid mapping in human dystrophic muscle by cluster-time-of-flight secondary ion mass spectrometry imaging
J. Lipid Res., February 1, 2008; 49(2): 438 - 454.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. G. Tidball and M. Wehling-Henricks
The role of free radicals in the pathophysiology of muscular dystrophy
J Appl Physiol, April 1, 2007; 102(4): 1677 - 1686.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. R. Beattie, C. Maguire, S. Gilchrist, L. J. Barrett, C. E. Cross, F. Possmayer, M. Ennis, J. S. Elborn, W. J. Curry, J. J. McGarvey, et al.
The use of Raman microscopy to determine and localize vitamin E in biological samples
FASEB J, March 1, 2007; 21(3): 766 - 776.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.