J. Lipid Res.
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A more recent version of this article appeared on August 1, 2005

Papers In Press, published online ahead of print June 1, 2005
J. Lipid Res., doi:10.1194/jlr.M500138-JLR200
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Submitted on April 8, 2005
Revised on May 10, 2005
Accepted on May 19, 2005

Lipid composition of integral purple membrane by 1H and 31P NMR

Christian Renner, Brigitte Keßler, and Dieter Oesterhelt

Bioorganic Chemistry, Max-Planck-Institut für Biochemie, Martinsried 82152

Corresponding Author: renner{at}biochem.mpg.de

In the purple membrane of halobacteria lipids stabilize the trimeric arrangement of bacteriorhodopsin molecules and mediate the packing of the trimers in a regular crystalline arrangement. Identification and quantification of these lipids has so far been based either on lipid extraction procedures or structural models. By directly solubilizing purple membranes from H. salinarum in aqueous detergent solutions (SDS or Triton-X100) we avoided any separation or modification steps that might modify the lipid composition or even modify the lipid molecules themselves. Our analysis of integral purple membrane preparations should resolve partially conflicting literature data on the lipid composition of the purple membrane. Using 31P and 1H NMR of detergent solubilized, but otherwise untreated samples we find two glycolipids and 6.4 +/- 0.1 phospholipids per bacteriorhodopsin molecule, 4.4 +/- 0.1 of the latter being the phosphatidylglycerophosphate methyl ester. The only glycolipid detected is S-TGD-1. For an additional glycolipid, GlyC, that was recently identified in lipid extracts, it could be shown, that it is produced mainly during the lipid extraction procedure, but partially also dependent on the preparation of the purple membrane suspensions.


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