Submitted on May 10, 2005
Revised on July 15, 2005
Accepted on July 21, 2005
Separation of polyprenol and dolichol by monolithic silica capillary column chromatography
Takeshi Bamba, Eiichiro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, and Akio Kobayashi
Department of Biotechnology, Osaka University, Suita, Osaka 565-0871
Corresponding Author: fukusaki{at}bio.eng.osaka-u.ac.jp
We attempted an analysis of naturally occurring polyprenol and dolichol using a monolithic silica capillary column in high-performance liquid chromatography (HPLC). First, the separation of the polyprenol mixture alone was performed using a 250 × 0.2 mm I.D. octadecylsilyl (ODS)-monolith silica capillary column. The resolution of the separation (Rs) between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded the level recorded when using a conventional ODS-silica particle-packed column (250×4.6 mmI.D.) under the same elution conditions by twice or more. Next, the mixture of the prenol type (polyprenol) and dolichol type (dihydropolyprenol) was subjected to this capillary HPLC system and the separation of each homologue was successfully achieved. During the analysis of polyprenol fraction derived from Eucommia ulmoides leaves, dolichols were found as a single peak including all-trans-polyprenol and cis-polyprenol previously identified. This high-resolution and sensitive system is very useful for the analysis of compounds which are structurally close to polyprenols and dolichols and which have a low content.
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