Submitted on May 12, 2005
Revised on July 12, 2005
Accepted on July 17, 2005
Relation between insulin resistance and fast-migrating low density lipoprotein subfraction as characterized by capillary isotachophoresis in non-diabetic children
Bo Zhang, Takuya Kaneshi, Takao Ohta, and Keijiro Saku
Department of Cardiology, Fukuoka University, Fukuoka 814-0180
Corresponding Author: bozhang{at}fukuoka-u.ac.jp
Objective: The proportion of the electronegative low density lipoprotein [LDL(-)] subfraction, which is atherogenic, is increased in type 2 diabetes, but is not reduced by glycemic control. Therefore, we evaluated the ability of a new technique, capillary isotachophoresis (cITP), to quantify charge-based LDL subfractions, and examined the relation between insulin resistance and the cITP fast (f)-migrating LDL levels. Methods and Results: Seventy-five 10-year-old boys were included. The two cITP LDL subfractions, fLDL and major LDL subfractions, were proportional to the LDL protein content within the range of 0.1 – 0.8 mg/ml LDL protein. Levels of cITP fLDL were positively correlated with triglyceride (TG) levels and negatively correlated with LDL size. Insulin resistance as assessed by the homeostasis model assessment (HOMA-IR) was positively correlated (p<0.01) with cITP fLDL levels (r=0.41). The relation between HOMA-IR and cITP fLDL levels depended on TG levels, but was independent of body mass index and LDL size. Conclusions: cITP lipoprotein analysis is an accurate and sensitive method for quantifying charge-based LDL subfractions in human plasma and insulin resistance is related to cITP fLDL independent of LDL size.
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