J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on February 1, 2006

Papers In Press, published online ahead of print November 29, 2005
J. Lipid Res., doi:10.1194/jlr.M500431-JLR200
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
M500431-JLR200v1
47/2/310    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ruberg, F. L.
Right arrow Articles by Hamilton, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruberg, F. L.
Right arrow Articles by Hamilton, J. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on September 29, 2005
Revised on November 8, 2005
Accepted on November 28, 2005

Identification of cholesteryl esters in human carotid atherosclerosis by ex vivo image-guided proton magnetic resonance spectroscopy

Frederick L. Ruberg, Jason Viereck, Alkystis Phinikaridou, Ye Qiao, Joseph Loscalzo, and James A. Hamilton

Department of Physiology and Biophysics, Boston Univeristy Medical Campus, Boston, MA 02118

Corresponding Author: jhamilt{at}bu.edu

Vulnerable atherosclerotic plaques may be identified by their large lipid component, particularly liquid cholesteryl ester (CE), covered by a fibrous cap. We hypothesized that image-guided (1H) proton magnetic resonance spectroscopy (MRS) would identify mobile CE in discrete, pre-selected regions of atherosclerotic plaque. Human carotid endarterectomy specimens (n=10) were imaged ex vivo by magnetic resonance imaging (MRI) at high-field (11.7 T) utilizing standard T1 and T2 weighted spin-echo protocols. Magnetic resonance spectroscopy (MRS) spectra were acquired from 1 mm3 voxels, localized to plaque regions that we judged by MRI to be lipid-rich or lipid-poor. The spectra revealed methyl and methylene resonances of fatty acyl chains with relative intensities and linewidths characteristic of pure CE by comparison with lipid standards. Regions judged to be lipid-rich by MRI showed much more intense CE resonances than lipid-poor regions. The integrated intensities of lipid peaks were 5.5%±2.0% (lipid-rich regions) vs. 0.9%±0.6% (lipid-poor regions) of the unsuppressed water peak (p<0.0001). Lipid distribution by histology, MR spectroscopy, and MR imaging showed strong correlation. Image-guided proton MRS accurately identified CE in selected regions of atherosclerotic plaque as small as 1 mm3 in an ex vivo setting. This procedure may permit the non-invasive detection and quantification of CE in atherosclerotic plaque in vivo.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.