J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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A more recent version of this article appeared on April 1, 2006

Papers In Press, published online ahead of print January 28, 2006
J. Lipid Res., doi:10.1194/jlr.M500506-JLR200
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Submitted on November 21, 2005
Revised on January 24, 2006
Accepted on January 28, 2006

LC-MS based method for the qualitative and quantitative analysis of complex lipid mixtures

Ulf Sommer, Haya Herscovitz, Francine K. Welty, and Catherine E. Costello

Biochemistry Dept., Boston Univ. School of Medicine, Boston, MA 02118-2526

Corresponding Author: cecmsms{at}bu.edu

A simple and robust LC-MS based methodology for the investigation of lipid mixtures is described and its application to the analysis of human lipoprotein-associated lipids is demonstrated. After an optional initial fractionation on Silica 60, normal-phase HPLC-MS on a YMC PVA-Sil column is employed first for class separation, followed by reversed-phase LC-MS or LC-MS/MS using an Atlantis dC18 capillary column, and/or nanospray MS, to fully characterize the individual lipids. The methodology is applied here for the analysis of human apolipoprotein B-associated lipids. This approach allows for the determination of even low percentages of lipids of each molecular species, and showed clear differences between lipids associated with apolipoprotein B100-LDL isolated from a normal individual and those associated with a truncated version, apolipoprotein B67-containing lipoproteins, isolated from a homozygote patient with familial hypobetalipoproteinemia. The methods described should be easily adaptable to most modern MS instrumentation.


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