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A more recent version of this article appeared on May 1, 2006

Papers In Press, published online ahead of print February 13, 2006
J. Lipid Res., doi:10.1194/jlr.M600001-JLR200
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Submitted on January 3, 2006
Revised on February 10, 2006
Accepted on February 12, 2006

Differentiation-associated expression of ceramidase isoforms in cultured keratinocytes and epidermis

Evi Houben, Walter M. Holleran, Toshiaki Yaginuma, Cungui Mao, Lina M. Obeid, Vera Rogiers, Yutaka Takagi, Peter M. Elias, and Yoshikazu Uchida

Department of Dermatology, Dermatology Service (190),, University of California San Francisco, San Francisco, CA 94121

Corresponding Author: uchiday{at}itsa.ucsf.edu

Ceramides accumulate within the interstices of the outermost epidermal layers, or stratum corneum, where they represent critical components of the epidermal permeability barrier. Although the stratum corneum contains substantial sphingol, indicative of ceramidase (CDase) activity, which CDase isoforms are expressed in epidermis remains unresolved. We hypothesized here that CDase isoforms are expressed within specific epidermal compartments in relation to functions that localize to these layers. Keratinocytes/epidermis express all five known CDase isoforms, of which acidic- and alkaline-CDase activities increase significantly with differentiation, persisting into the stratum corneum. Conversely, neutral- and phytoalkaline-CDase activities predominate in proliferating keratinocytes. These differentiation-associated changes in isoform activity/protein are attributed to corresponding, differentiation-associated changes in mRNA levels (by quantitative RT-PCR). Although four of the five known CDase isoforms are widely expressed in cutaneous and extracutaneous tissues, alkaline CDase-1 occurs almost exclusively in epidermis. These results demonstrate large, differentiation-associated and tissue-specific variations in the expression and activities of all five CDase isoforms. Since alkaline CDase-1 and acidic CDase are selectively up-regulated in the differentiated epidermal compartment, they could regulate functions that localize to the distal epidermis, such as permeability barrier homeostasis and antimicrobial defense.


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