Submitted on January 20, 2006
Revised on July 17, 2006
Accepted on July 30, 2006
Endothelial lipase is less effective in influencing HDL metabolism in vivo in mice expressing apoA-II
Uli C. Broedl, Weijun Jin, Ilia V. Fuki, John S. Millar, and Daniel J. Rader
Department of Internal Medicine II, University of Munich, Munich 81377
Corresponding Author: uli.broedl{at}med.uni-muenchen.de
Endothelial lipase (EL) plays an important physiological role in modulating high density lipoprotein (HDL) metabolism. Data suggest that plasma contains an inhibitor of EL and previous studies have suggested that apoA-II inhibits the activity of several enzymes involved in HDL metabolism. We therefore hypothesized that apoA-II may reduce the ability of EL to influence HDL metabolism. In order to test this hypothesis, we determined the effect of EL expression on plasma phospholipase activity and HDL metabolism in human apoA-I and human apoA-I/A-II transgenic mice. Expression of EL in vivo resulted in lower plasma phospholipase activity and significantly less reduction of HDL cholesterol, phospholipid and apoA-I levels in apoA-I/A-II double transgenic mice compared with apoA-I single transgenic mice. We conclude that the presence of apoA-II on HDL particles inhibits the ability of EL to influence the metabolism of HDL in vivo.
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