High density lipoprotein subfractions: isolation, composition and their duplicitous role in oxidation

Peter A. C. McPherson, Ian S. Young, Bronac McKibben, and Jane McEneny

Nutrition and Metabolism Group, Queen's University Belfast, Belfast, County Antrim BT12 6BJ

Corresponding Author: j.mceneny{at}qub.ac.uk

The plasma HDLs are a major class of cholesterol transporting lipoprotein which can be divided into two distinct subfractions, HDL2 and HDL3, by ultracentrifugation. Existing methods for the subfractionation of HDL requires lengthy ultracentrifugations, making them unappealing for large scale studies. We describe a method which subfractionates HDL from plasma in only 6 hours, representing a substantial decrease in total isolation time. The subfractions so isolated were assessed for a variety of lipid and protein components, in addition to their susceptibility to oxidation, both alone and in combination with VLDL and LDL. We report for the first time a pro-oxidant role for HDL during VLDL oxidation, where HDL is donating preformed hydroperoxides to VLDL in a CETP-dependent process. Examination of the participation of HDL in LDL oxidation has reinforced its classic role as a potent antioxidant. Furthermore, we have also implicated the second major HDL-associated enzyme LCAT in these processes, whereby it acts as a potent pro-oxidant during VLDL oxidation, but as an antioxidant during LDL oxidation. Thus, we have identified a potentially duplicitous role for HDL in the pathogenesis of atherosclerosis, due to both CETP and LCAT.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

K. E Bradbury, C M. Skeaff, T. J Green, A. R Gray, and F. L Crowe
The serum fatty acids myristic acid and linoleic acid are better predictors of serum cholesterol concentrations when measured as molecular percentages rather than as absolute concentrations
Am. J. Clinical Nutrition, February 1, 2010; 91(2): 398 - 405.
[Abstract] [Full Text] [PDF]

J. D. Smith
Dysfunctional HDL as a Diagnostic and Therapeutic Target
Arterioscler Thromb Vasc Biol, February 1, 2010; 30(2): 151 - 155.
[Abstract] [Full Text] [PDF]

V. Y. Fujimoto, J. P. Kane, B. Y. Ishida, M. S. Bloom, and R. W. Browne
High-density lipoprotein metabolism and the human embryo
Hum. Reprod. Update, January 1, 2010; 16(1): 20 - 38.
[Abstract] [Full Text] [PDF]

A. M. Vincent, J. M. Hayes, L. L. McLean, A. Vivekanandan-Giri, S. Pennathur, and E. L. Feldman
Dyslipidemia-Induced Neuropathy in Mice: The Role of oxLDL/LOX-1
Diabetes, October 1, 2009; 58(10): 2376 - 2385.
[Abstract] [Full Text] [PDF]

L. C. Tsoi, M. Boehnke, R. L. Klein, and W. J. Zheng
Evaluation of genome-wide association study results through development of ontology fingerprints
Bioinformatics, May 15, 2009; 25(10): 1314 - 1320.
[Abstract] [Full Text] [PDF]

R.W. Browne, W.B. Shelly, M.S. Bloom, A.J. Ocque, J.R. Sandler, H.G. Huddleston, and V.Y. Fujimoto
Distributions of high-density lipoprotein particle components in human follicular fluid and sera and their associations with embryo morphology parameters during IVF
Hum. Reprod., August 1, 2008; 23(8): 1884 - 1894.
[Abstract] [Full Text] [PDF]